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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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The choice between them affects stability and storage duration, not the calculation

3-D cell discs were formed by culturing vascular smooth muscle cells in collagen first and then 3-D discs with similar size were incubated in different media for 24 h

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