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Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)

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Urbano T, Vinceti M, Mandrioli J, Chiari A, Filippini T, Bedin R, Tondelli M, Simonini C, Zamboni G, Shimizu M, Saito Y

This transcriptional activation provides sustained protection against oxidative stress by increasing cellular antioxidant enzyme capacity