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10.1074/jbc.M410818200
The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
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any change still requires FDA rulemaking for the individual compounds
The peptides potential role in connective tissue healing has attracted significant scientific attention since the late 1990s and early 2000s, with research published in journals focused on orthopedic surgery, sports medicine, and pharmacology
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