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This process, along with GCL inhibition and the formation of mixed disulfides between GSH and protein sulfhydryl, concurred to a steady-state decrement of free GSH concentration and, consequently, to an intracellular redox state shift toward more oxidizing conditions, which could be required for avoiding fast reduction of oxidized cysteines allowing the upstream ROS-induced autophagic stimulus to be propagated
However, for long-term storage (anything more than a few weeks), it must be kept in a freezer, ideally at -20C (-4F)
Total proteins were immunoblotted using an -HA antibody for detecting SUMM2 ac -HA (upper panel) and an -FLAG antibody for LET7-FLAG, LET7 TPR -FLAG, and LET7 PPase -FLAG (middle panel) with Ponceau S-stained RBC as loading controls (bottom panel)
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Keep in a cool, dry place, tightly capped and out of the reach of children._END_SUGGESTED_USE_
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