The injection itself is typically painless, using a fine 29-gauge needle similar to insulin pens
Aggressive shaking creates air bubbles and can denature peptides through a process called interfacial stress, where the peptide is damaged at the air-liquid interface
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Two chloroplastic, one thylakoid-bound, and one membrane-bound APX, whose product is targeted to both chloroplast stroma and mitochondria, were identified in the model plant Arabidopsis thaliana , while the intracellular localization of an additional member is still unknown (Najami et al., 2008)
GSTs also show thiol transferase activity and have a role in thiolysis and isomerization reactions 1
10.1016/j.biotechadv.2018.03.017 150 KimI