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Due to its very low ability to synthesize and regenerate GSH, the brain is one of the most vulnerable tissues to excess ROS 15,21

Titin phosphorylation status (Ser4080 by phosphosite-specific antibody western blot, pro-Q Diamond gel stain) and N2B/N2BA isoform ratio (SDS-agarose gel, titin isoform migration) determine passive stiffness mechanisms

In addition to its effects on neurotransmitters, the sleep peptide influences hormonal activity by lowering ACTH levels, which reduces stress-related cortisol production, and by enhancing growth hormone (GH) secretion, essential for restorative sleep