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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C
doi: 10.1080/17425255.2021.1925249
J Am Acad Child Adolesc Psychiatry
Milk thistle in liver diseases: Past, present, future
Potential side effects could range from mild to severe, including allergic reactions, injection site complications, and systemic effects
10.3389/fphys.2014.00415 73 SchechterA
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