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Importantly, overexpression of the wild-type (WT) AMPK1 catalytic subunit in shGDH1 cells not only increased cytosolic NADPH level and alleviated disulfide stress (Fig

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Subsequently, 50 l of TUNEL reagent mix was applied to each slide, followed by incubation at 37 C for 60 min in a humidified chamber, as per the manufacturers instructions