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Thus, cystine limitation suppressed mTORC1 and activated the ISR, the two major amino acid sensing pathways in HepG2 cells

If it happens, rinse it with fresh water and let it air dry

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The 4-month rest is not optional

The next day, the supernatant was decanted into a 15-mL sterile conical centrifuge tube and centrifuged at 400 g for 10 min at 4 C

Quantitative analysis of autophagic flux by confocal pH-imaging of autophagic intermediates