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It also contains L-glutamic acid, L-cystine and glycine, which are the amino acids making up L-glutathione
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Figure 2)
The cells were lysed in a Buffer A (50 mM Tris- HCl, pH 7.5, 500 mM NaCl, 10 mM imidazole, protease inhibitor cocktail (Roche)) and subjected to sonication
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