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Plates were subsequently washed with 0.1% polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline (TTBS), immediately fixed with cold 3.7% paraformaldehyde in PBS, and re-treated with the blocking solution overnight at 4C

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Rinse Rinse with cool water to seal the cuticle and lock in moisture

Other treatment options may include metformin , a diabetes medication that may be prescribed off-label for fatty liver disease

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