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At 48-hour post-treatment, the cell culture supernatant was collected and tested on Human IFN gamma ELISA Kit (Invitrogen), Human IL-2 ELISA Kit (Acro Biosystems), and Human TNF alpha Uncoated ELISA Kit (Invitrogen) to measure IFN-, IL-2 and TNF-, respectively, according to the manufacturer instruction
J., Iavarone, A
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One randomized controlled trial demonstrated that choline supplementation (750 mg/day of choline in the form of phosphatidylcholine administered from week 18 of gestation through 90 days postpartum) in pregnant women consuming a moderate-choline diet (approximately 360 mg/day) was safe but did not enhance infant cognitive function at 10 or 12 months of age (215)
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