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CRISPR drop out assay Lentiviruses were produced in HEK293T cells by transfection of plasmids, containing the guideRNA and an RFP gene for the readout, with Lipofectamine TM 2000 transfection reagent

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(1997) J Mol Biol 274 : 84-100 PubMed: 9398518 Search on PubMed DOI: Primary Citation Related Structures: 10GS, 5GSS, 6GSS, 7GSS, 8GSS, 9GSS PubMed Abstract: The human pi-class glutathione S-transferase (hGST P1-1) is a target for structure-based inhibitor design with the aim of developing drugs that could be used as adjuvants in chemotherapeutic treatment

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the goal is to steadily replenish deep tissue stores over weeks and months, slowly shifting the cellular environment from a state of emergency to a state of repair

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The search was limited to articles published in English, with a focus on recent studies (20102024) while including seminal older works for historical context

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If injections are stopped when the cause of B12 deficiency has not been treated, symptoms will return, seriously affecting the patient

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(D) Schematic of glutathione metabolism, along with a dot plot visualizing genes involved in glutathione metabolism, displaying average expression and percentage expressed in PEC and IC-B cells from ABMR (red) and healthy kidney samples (black)

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