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The tissue was homogenized thrice at 1500 rpm for 120 s using a tissue homogenizer (Micro Smash MS100R, Tomy Digital Biology Co., Ltd., Tokyo, Japan) following which the homogenate was centrifuged at 2300 g and 4 C for 5 min

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IV therapy allows fluids and selected nutrients to be administered intravenously under trained clinical supervision

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Presented for research literature review only Guzmn-Guilln R et al