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The culture medium consisted of Dulbeccos Modified Eagle Medium/F12 (DMEM/F12, Hyclone, SH30023.01B, USA) and -Minimum Essential Medium (-MEM, Hyclone, SH30265.01B, USA), supplemented with 0.23 mmol/L sodium pyruvate (Hyclone, SH40003-12, USA), 10% Fetal Bovine Serum (FBS
Moreover, theres increasingly more evidence linking vitamin B12 deficiencies to brain, heart, inflammatory, and immune system disorders (4)
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These intracellular pathways control transcriptional events related to growth hormone synthesis and release
G., Casals, N., and Champion, M
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