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The Gln uptake ratio is calculated as (C1-C2)/Cell count, where C1 is the initial concentration of Gln, and C2 is the concentration of Gln in the medium after 24 h of incubation
It also matters for accuracy at high doses, because you can fit the entire dose within a 1 ml syringe up to 20 mg
(PubMed) Estrada DE, Ewart HS, Tsakiridis T, et al
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