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Alternative volumes for different final concentrations Rule of thumb: For NAD+ we recommend 5 mL volume for a 250 mg vial (50 mg/mL)

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2021YFC2100300) and the National Natural Science Foundation of China (Grant No

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Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36