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doi:10.1172/JCI112855 8
Association of bitter taste receptors with obesity and diabetes and their role in related tissues
The obesity-drug revolution is stalling
mRNA expression was analyzed using a single-step reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR) using a ViiA 7 Real-Time PCR System (Life Technologies) using Taqman fast PCR Master Mix and TaqMan primer and fluorescent probe sets (listed in Tables S2 and S3) for 20 s at 95 C, then 40 cycles at 95 C (1 s) and 60 C (20 s)
Journal of Diabetes and its Complications, 34 (11), 107704
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