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To identify potential off-target substrates, we searched enriched motifs against the human proteome, using the enrichment score of the IL-1 motif (cleavage between Glu 18 and Asp 19) as the cutoff for predicted cleavage activity (Fig
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Moreover, the expression of signal transducer and activator of transcription 5A ( STAT5A) , an essential mediator of IL-2 signaling in T-cells ( Figure 6 Most intriguingly, the expression of T H 17-related genes such as IL17A, IL17F, IL22 , and B-cell activating transcription factor (BATF) was strongly decreased by SFN, while T H 1, T H 2 or Treg related genes, i.e., STAT4, IL-4 , and FOXP3 were no major targets of SFN (Figure 6C)
