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Tetrahydrofuran was distilled over sodium benzophenone ketyl and CH 2 Cl 2 was distilled over phosphorus pentoxide
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doi: 10.3389/fpls.2015.01272 46 SinghA
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Briefly, enzyme activity was determined at 37C in reaction mixtures of 1.0 ml containing 100 mm Tris-HCl buffer (pH 8.2), 150 mm KCl, 5 mm ATP, 2 mm phosphoenolpyruvate, 10 mm glutamate, 10 mm -aminobutyrate, 20 mm MgCl 2 , 2 mm EDTA, 0.2 mm NADH, 17 g pyruvate kinase, and 17 mg lactate dehydrogenase
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