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doi: 10.1038/s41580-018-0093-z 29 VarolCZvibelISpektorLMantelmacherFDVugmanMThurmTet al

Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

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