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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

BPC-157, a peptide known for its regenerative properties, typically begins to show noticeable effects within a few days to a week after starting treatment, though the timeline can vary depending on the severity of the injury and the individuals response

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