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For longer storage, freeze single-use aliquots at minus 20C and avoid repeated freeze-thaw cycles, which break the molecule down

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10.1093/oxfordjournals.jbchem.a133393 101 ZainolA

Vitamin B12 is a water-soluble vitamin that acts as a cofactor for methionine synthase and methylmalonyl-CoA mutase, supporting nerve function, red blood cell production, and DNA synthesis