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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )

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It has been linked to increased energy production, improved sleep quality and reduced inflammation

Transcriptional profiling shows altered expression of wnt pathwayand lipid metabolismrelated genes as well as melanogenesis-related genes in melasma

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There is no reversal process