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We further validated the nominated substrates by expressing full-length proteins in HEK293T cells and incubating lysate with 1 M X(PC) and BoNT/X protease (Extended Data Fig

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In skeletal muscle, Akt promotes glucose uptake via the translocation of glucose transporter type 4 (GLUT4) storage vesicles (GSVs) to the plasma membrane, which is mediated by inactivation of GTPase-activating protein (GAP) AKT substrate of 160 kDa (AS160) and promotion of GTP-bound form of Ras-related C3 botulinum toxin substrate 1 (RAC1)

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