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After washing in PBS/BSA, the O/PEs were mounted on slides using Vectashield (H-1000-10, Vector Laboratories, Newark, CA, USA)

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This is because the sustained activation of PI3K-AKT pathway inhibits GSK3, which phosphorylates NRF2 and allows its recognition and ubiquitination by TrCP-CUL1 ubiquitin E3 ligase for degradation

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The Effectiveness of Intermittent Fasting to Reduce Body Mass Index and Glucose Metabolism: A Systematic Review and Meta-Analysis

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