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Easy measuring: At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg (0.05 mg) on a U-100 insulin syringe

Regular IGF-1 has 70 amino acids, while Long R3 IGF-1 has 83 amino acids due to the addition of 13 amino acids on its N-terminal end

The animals were assigned to six experimental groups (n = 7 per group) using stratified randomization to balance body weights across groups at baseline

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