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Figure 3 Superoxide does not oxidize protein thiols at a relevant pace In a previous study we did not detect oxidation of N-acetylcysteine, a cysteine analog, when it was incubated in oxic buffer (Eben and Imlay, 2023)
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This mechanistic synergy, first characterised in foundational studies of GHRH and GHRP co-administration, provides the physiological and pharmacological rationale for the combination as a research tool for studying maximal physiological GH pulse generation and GH axis pharmacology
The supernatants were incubated with Protein A/G Dynabeads pre-washed with ice-cold PBS and coupled to the respective antibodies