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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

Even determining which form of the peptides should be used was unclear to regulators, since substances like TB-500 aren't recognized as official pharmaceutical ingredients in the U.S

The most commonly reported issue is increased energy or excitability in the first couple of weeks