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After the treatment period, cells were fixed in 4% paraformaldehyde, permeabilized using 90% methanol, and incubated at room temperature for 1 h with primary antibodies against GPX4 and SLC7A11 (1:2000)
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Reitblat, O
Nutrients , 15 (13), 3073
Barrozo LG, Silva BR, Paulino L, Barbalho EC, Nascimento DR, Costa FC, Batista A, Lopes EPF, Rodrigues APR, Silva JRV (2022) N-Acetyl cysteine reduces the levels of reactive oxygen species and improves in vitro maturation of oocytes from medium-sized bovine antral follicles
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