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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)
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10.1016/j.envpol.2018.03.006 11 BiffiA.CantaruttiA.ReaF.LocatelliA.ZaniniR.CorraoG
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To ensure optimal staining conditions, the slides were subjected to three consecutive washes with phosphate-buffered saline (PBS), each lasting five minutes
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