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Be patient: This usually takes 2 to 5 minutes of consistent swirling, mixing in a circular motion, and gently flipping the vial up and down

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For metabolite extraction, 5 l of urine was transferred to a glass insert and extracted using 195 l of cold 80% methanol spiked with 500 nM metabolomics amino acid standard mix (Cambridge Isotopes Laboratory)

Aliquot for Storage (Optional): To ensure stability and avoid repeated thawing, you can apportion the reconstituted solution into smaller, single-use or weekly-use aliquots in separate sterile vials

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