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Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG)

Once LCMS analysis was performed, Skyline software was used for metabolite identification

doi: 10.11622/smedj.2018043 133 CohenSJanicki-DevertsDMillerGE

As a follow-up, an FDA roadmap has been published, stating that in 35 years time human-relevant testing methods (non-animal New Approach Methods) will be the default for safety/toxicity testing instead of animal studies (218)

There is no identified upper limit for B12 as it's a water-soluble nutrient, meaning any excess is removed in urine