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Raw fluorescence values were corrected for background fluorescence, which was measured in wells without cells containing only medium, one-step assay buffer and test compound
2009, 4: e4512- 10.1371/journal.pone.0004512 Lo R, Turner MS, Barry DG, Sreekumar R, Walsh TP, Giffard PM: Cystathionine -lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11
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Free, uncomplexed GHK and excess copper salts can also appear
ROS oxidize DCF to the fluorescent product fluorescein
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