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To construct shRNAs, forward oligonucleotides were designed to contain the sense and antisense sequences connected with a hairpin loop (5-CTGTGAAGCCACAGATGGG-3) followed by a poly(dT) termination signal
Importantly, tricyclic antidepressants, especially amitriptyline and nortriptyline, worsen the metabolic profile, increasing insulin resistance, contributing to weight gain and causing hyperglycemia, thereby increasing the risk of diabetes with prolonged use [77]
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