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SDS-PAGE, Immunoblotting, and mass spectrometry Equal cell numbers from liquid-grown cultures or equal amounts of CsCl fractions were solubilized in 2 Tris-Glycine SDS buffer (Life Technologies) by boiling for 15 min or heating at various temperatures
In this exemplary reaction scheme, coupling agent EDCI is added to Boc-homoarginine
Bibcode:2016NatSR...637111C
In order to function in spatio-temporally controlled signaling events, most of these reactions need to be catalyzed by enzymes to reach the required reaction velocities and specificities
David Fisher, The New England Journal of Medicine , any significant skin pigmentation change should be examined, especially in patients with high-risk factors for skin cancer
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