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To infer a functional relationship between these conserved residues and APX sequences, we searched for the known binding residues of model organism Arabidopsis APXs in the UniProtKB database ( The following residues were reported as potential active and metal binding residues for Arabidopsis GPX1-6, APXT, and APXS: APX1 (Arg-38, His-42, His-163, Thr-164, Thr-180, Asn-182, Ile-185, Asp-187), APX2 (Arg-39, His-43, His-163, Thr-164, Thr-180, Asn-182, Ile-185, Asp-187), APX3 (Arg-36, His-40, His-160, Thr-161, Thr-177, Asp-184), APX5 (Arg-35, His-39, His-158, Thr-159, Thr-175, Asp-182), APX6 (Arg-119, His-123, His-224), APXT (Arg-108, His-112, His-241, Thr-242, Thr-274, Asp-281), and APXS (Arg-129, His-133, His-262, Thr-263, Thr-295, Asp-302)
It's typically prepared at 0.1 to 1.0% acetic acid concentration, yielding a pH between 3.0 and 4.5
August 9, 2022
2006 Dec;14(5-6):214-21
Croft AP, Campos J, Jansen K et al (2019) Distinct fibroblast subsets drive inflammation and damage in arthritis
Cheeseman KH, Holley AE, Kelly FJ, Wasil M, Hughes L, Burton G
