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Significant findings and lessons were learned from this pilot phase

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Immunofluorescence tissues were mounted with Immuno-Mount (Thermo Fisher, 9990402) and coverslipped for imaging

Vitamin C mechanisms involve serving as enzymatic cofactor for collagen hydroxylation and providing direct antioxidant protection, creating effects requiring ongoing application for maintaining antioxidant benefits but potentially supporting durable collagen improvements if used sufficiently long

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