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Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA
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Rogan first learned about Lions Mane from mushroom expert Paul Stamets in JRE #1035
133 (11 Suppl 2): 3972S3980S
10.1080/10715762.2017.1379602 Free Radic Res
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