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After treatment, the cells (2106/ml) were incubated with 10 M/l DCFH-DA for 20 min at 37C in the dark, washed three times with PBS and were then observed under a fluorescence microscope (Nikon Eclipse ti2

In the present study, GLP-1R expression in human endothelial cells was detected by RT-PCR and western blot analyses, and we demonstrated that GLP-1R at both mRNA and protein levels was detectable, albeit not very strong, in the human endothelial cells

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Higher doses produce more pronounced GLP-1 receptor activation, resulting in greater delays in gastric emptying and more intense central effects