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The following antibodies were used: anti-GAPDH (32233, Santa Cruz Biotechnology), rabbit anti-FLAG (F7425, Sigma-Aldrich/Merck) or M2 (F3165, Sigma-Aldrich/Merck), anti-LC3B (L7543, Sigma-Aldrich/Merck), anti-WIPI2 (purified mouse IgG clone 2A2 from the Tooze lab), anti-GFP (A11122, Thermo Fisher Scientific), anti-C16orf70 (ab181987, Abcam), anti-ATG16L1 (D6D5, Cell Signaling Technology), antiBeclin 1 (D40C5, Cell Signaling Technology), anti-ATG7 (D12B11 Cell Signaling Technology), anti-HA (C29F4, Cell Signaling Technology), and anti-BCAS3 (ab71162, Abcam)
Janku F, De Groot JF, Javle MM, Penas-Prado M, Shroff RT, Luthra R, Banks K, Lanman RB, Conley AP, Broaddus R, Davies MA, Kopetz S , Yung WKA, Heymach J, Fu S, Shaw KR, Meric-Bernstam F
These findings are consistent with previous reports that BoNT/F light chain cleaves only VAMP1, VAMP2 and VAMP3, while BoNT/X cleaves VAMP1 through VAMP5 as well as YKT6 (refs
A free weight squat with a barbell takes a lot of balance and coordination
Alternative approaches to GLP-1 weight loss Telehealth providers like Elevate Health represent just one path to GLP-1 medications
Injection site selection (abdomen, thigh, or upper arm) does not significantly alter absorption rates or overall bioavailability, providing flexibility for patient preference and rotation strategies to minimize injection site reactions
