Secondary antibodies and their dilutions were as follows: Rhodamine (TRITC)-AffiniPure Donkey Anti-Goat IgG (H + L) antibody (1:1000, Jackson ImmunoResearch Labs Cat# 705025003, RRID:AB_2340388), Donkey Anti-Mouse IgG (H + L), and Alexa Fluor647-conjugated polyclonal antibody (1:1000, Thermo Fisher Scientific Cat# A-31571, RRID:AB_162542)
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