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In the absence of protein cleavage, the high effective concentration of T7 lysozyme with respect to T7 RNAP ensures minimal RNAP activity
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The analysis of semaglutide after synthesis was analyzed using ultra-high performance liquid chromatography-UV-mass spectrometry (UPLC-UV-MS) on a BEH130 C4 column with a mobile phase containing acetonitrile, water, and 0.1% trifluoroacetic acid [91]
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