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Pulldown assay Wild-type (WT) and mutant GSTs were expressed in Rosetta (DE3) competent cells by 1 mM isopropyl -D-1-thiogalactopyranoside (IPTG) (Amnesco)
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Many protocols follow a 5-days-on, 2-days-off schedule to prevent receptor desensitization
Young PA, Senkal CE, Suchanek AL, Grevengoed TJ, Lin DD, Zhao L, et al
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