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Chronic alcohol consumption increases the number of recruited macrophages in the liver, promoting the differentiation of Ly6C hi monocytes into pro-inflammatory macrophages that contribute to tissue damage [47]
For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
In: NCT02663570, C.g.I
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