M.Niso-SantanoM
Pharm Dev Technol 22:370382 Eng J, Kleinman WA, Singh L, Singh G, Raufman J-P (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom
The GST-tagged protein was trapped on the column and purged using a running buffer (200 mM NaCl and 50 mM Tris-HCl at pH 7.8) before being eluted with 200 mM NaCl, 50 mM Tris-HCl and 10 mM reduced glutathione at pH 7.8
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Nature Lee E, Walker C, Ayadi B
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