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Upon removal of the supernatants, the final AuNP-anti-B 12 conjugates were reconstituted in 0.01 M TBS containing 2% bovine serum albumin (BSA) and the final optical density (O.D.) was checked using Spectramax 384 at 530 nm

It is not approved for human or veterinary use, therapeutic applications, or diagnostic procedures

These would provide steady drug levels without weekly injection burden

These figures come from commonly cited reference protocols, not approved human dosing and human weight-loss efficacy has not been demonstrated [3] [4]

Future research should focus on refining these treatment modalities and expanding comparative studies to optimize B12 deficiency management strategies effectively