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A consistent finding across multiple studies is the identification of key molecular targets that influence macrophage polarization

Consequently, amino acid precursors involved in GSH synthesis and substances like N-acetylcysteine could enhance the effectiveness of GPX3 (44)

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Biochemical Society transactions 10 , 7879 (1982)

The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)