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Cysteine requires two more enzymatic reactions before being finally converted into glutathione

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Adeno-associated virus 9 construction of NR4a1 overexpression or knockdown mouse model To specifically knock down NR4a1 in cardiomyocytes, adeno-associated virus 9 (AAV9) carrying short hairpin NR4a1 (AAV9-shNR4a1) with cTNT as the promoter and its control vector (AAV9-shRNA) were constructed by Design Gene Biotechnology (Shanghai, China)