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Samples were fractionated by SDS-PAGE in Mini-PROTEAN TGX Precast Gels, 415% (Bio-Rad), and eletro-transferred onto nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad)

Meanwhile, microdose GLP-1s kickstart the action-motivation flywheel, enabling early successes by limiting food noise and creating space for healthier habits

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may extend to 16 weeks based on individual goals